Evidence that mismatched bases in heteroduplex T4 bacteriophage are recognized in vivo
نویسندگان
چکیده
منابع مشابه
Heteroduplex heterozygotes in bacteriophage T4 involving mutations of various dimensions.
Single-factor genetic crosses in bacteriophage T4 produce two distinct types of heterozygous progeny.' One kind probably corresponds to a terminal redundancy of the chromosome. The other kind, variously termed a heteroduplex heterozygote, an internal heterozygote, or a recombinational heterozygote, arises as an intermediate in recombination (Fig. 1). Internal heterozygotes are preferentially ac...
متن کاملEvidence for a complex regulating the in vivo activities of early enzymes induced by bacteriophage T4.
An in vivo assay of enzyme activity has been employed to investigate the mechanism of control of enzymes synthesizing deoxyribonucleotides after bacteriophage T4 infection. The assay is based on the release of tritium from Slabeled pyrimidine nucleotide substrates into water. At least two phage-induced early enzymes, deoxycytidylate hydroxymethylase and thymidylate synthetase, which are synthes...
متن کاملMisrepair mutagenesis in bacteriophage T4.
The T4 mutations px, y and 1206 inactivate an error-prone recombination-like repair system, reducing or abolishing mutagenesis by UV irradiation, MMS, and white light irradiation in the presence of the photosensitizer 8MOP. Both px and y increase some spontaneous mutation rates and slightly enhance proflavin mutagenesis; neither mutation affects thymineless or 2AP mutagenesis appreciably, but b...
متن کاملOld phage, new insights: two recently recognized mechanisms of transcriptional regulation in bacteriophage T4 development.
The regulation of bacteriophage T4 middle and late gene expression involves previously unrecognized mechanisms. Middle transcription requires a DNA-binding transcriptional activator and a sigma 70-binding co-activator. The coupling of late transcription to DNA replication is effected by a DNA-tracking protein that is loaded onto DNA by an assembly factor at enhancer-like entry sites. Late trans...
متن کاملAn immunoblot assay reveals that bacteriophage T4 thymidylate synthase and dihydrofolate reductase are not virion proteins.
Numerous reports describe the phage T4 enzymes thymidylate synthase and dihydrofolate reductase as structural components of the baseplate. However, Y. Wang and C. K. Mathews (J. Virol. 63:4736-4743, 1989) reported that antisera against the respective recombinant enzymes failed to neutralize phage infectivity, in contrast to previous results. Moreover, a deletion mutant lacking the genes for the...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1976
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.20.2.441-445.1976